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离心机是蛋白表达通关装备之一

发布时间:2020-12-17 14:38:21      点击:

  想要通关蛋白表达少不了离心机。常言道细节决定成败,尤其像蛋白表达提纯这样复杂的实验。一个操作上的小失误,可能直接影响最终结果。

  SUIRANKEYIDAZHIFENWEISANBU,BIAODA、FENLI、CHUNHUA,DANSHIZHEIGEGUOCHENGSHIFENFANSUO。

  DANBAIBIAODAQIANQIHAIYOUYIXILIEFUZAGUOCHENG,HAOFEISHIJIANYEZHANG,DAOZUIHOUYIBU,HAIBUNENGFANGSONGJINGTI。

  DANBAIJINXINGCHUNHUASHITONGGUANDEZUIHOUYIBU,XUANZEHESHIDECHUNHUAFANGFA,KEYITIGAODANBAIDECHUNHUAXIAOLV,RANGDANBAICHUNHUAJIANDANBIANJIE。CHANGYONGDEFANGFAYOU:QINHECENGXI、LIZIJIAOHUANCENGXI、NINGJIAOGUOLVCENGXI、SHUSHUICENGXIDENG。

DL-5M-新.jpg

  QINHECENGXI

  QINHECENGXICHUNHUASHILIYONGSHENGWUDAFENZIWUZHIJUYOUYUMOUXIEXIANGYINGDEFENZIZHUANYIXINGKENIJIEHEDETEXINGJINXINGDANBAICHUNHUADEJISHU。

  LIZIJIAOHUANCENGXI

  LIZIJIAOHUANCENGXISHIYIZHONGYIJUDANBAIBIAOMIANSUODAIDIANHELIANGBUTONGJINXINGDANBAIFENLICHUNHUADEJISHU。

  NINGJIAOGUOLVCENGXI

  NINGJIAOGUOLV(YEJIAOPAIZUCENGXIHUOFENZISHAI)SHIYIZHONGGENJUFENZIDAXIAOCONGHUNHEWUZHONGFENLIDANBAIZHIDEFANGFA。

  SHUSHUIZUOYONGCENGXI

  SHUSHUIZUOYONGCENGXISHILIYONGYAN-SHUITIXIZHONGYANGPINFENZIDESHUSHUIJITUANHECENGXIJIEZHIDESHUSHUIPEIJIZHIJIANSHUSHUILIDEBUTONGERJINXINGFENLIDEYIZHONGCENGXIFANGFA。

  SUIRANFANGFADUOZHONGDUOYANG,DANSHI,DOUXUYAOYONGLIXINJILAIJINXINGFENLICHUNHUA。

  YONGZHIFANGYANGHEMEIZHONGZUDANBAIDEYOUDAOWEILI:

  1.JIEZHONGHANYOUZHONGZUZHIFANGYANGHEMEIDANBAIDEDACHANGGANJUNBL21JUNZHUYU5 mL LBYETIPEIYANGJIZHONG(HAN100 ug/mL ANBIANQINGMEISU),37℃ZHENDANGPEIYANGGUOYE。

  2.AN1∶50HUO1:100DEBILIXISHIGUOYEJUN,YIBANZHUANJIE1 mLGUOYEPEIYANGWUYU100 mL(HAN100 ug/mL ANBIANQINGMEISU)LBYETIPEIYANGJIZHONG,37℃ZHENDANGPEIYANGZHIOD600 = 0.6 - 0.8(ZUIHAO0.6,DAYUEXU3 h)。QU10 ul YANGPINYONGYUSDS-PAGE FENXI。

  3.DUIZHAOZUBUJIAYOUDAOJI,SHIYANZUJIARUIPTGZHIZHONGNONGDU0.5 mmol/l,37℃JIXUPEIYANG1-3h。

  4.LIXINJI12000 rpm LIXIN10 min,QISHANGQING,JUNTICHENDIANBAOCUNYU-20℃HUO-70℃BINGXIANGZHONG。

  YISHANGJIUSHIGUANYUDANBAIBIAODADETONGGUANJIANGJIE,BENWENZHONGDIANJIUSHIYAOBIAODA,YIGESHIYANDECHENGBAI,XIJIEHENGUANJIAN,SUIRANRENGONGFANGMIANSHIKEYIRENWEIBAKONGDE,DANSHIFUZHUSHEBEIDEXIJIE,HAISHIYAODUOJIAZHUYI,XUANYITAIHESHIDELIXINJIHENGUANJIAN。


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