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质粒DNA提取不管小提大提都离不开它

发布时间:2020-12-9 17:48:12      点击:

  离心机在质粒DNA提取中有着很重要的地位,而且操作流程复杂。

  YINWEIZHILIDNAZUOWEIJIYINZHONGZUYUJIYINZHUANYIDEZAITI,ZAIYICHUANGONGCHENGYANJIUZHONGHENZHONGYAO。

  SUOYIZAIRICHANGDEXIBAOSHIYANZHONGJINGCHANGHUIYONGDAOZHILIDNA,XIANZAIDOUYONGJIHEFEICHANGFANGBIAN,ERQIEJUNTIPEIYANGHOU,KEYIDUOGUANNONGSUOTIQU,TIDAODEZHILILIANGDUO,KEYI-20℃BAOCUN,YIHOUYONGYUMEIQIE、ZHUANHUADENGSHIYAN,KEYITIQIANPAOGEJIAO,ZHIYAOBUJIANGJIE,JIUKEYIJIXUYONG,BIMIANMEICIYAOYONG,DOUYAOPEIYANGYIBIANZAITIQUYIBIAN。

TGL-16M-新.jpg

  ZHILIDNACHANGJIANDETIQUFANGSHIFENWEILIANGZHONG:

  YI.ZHILIXIAOTI

  JIANGZHIQIANZHUNBEIDELBYETIPEIYANGJIZHONGJIARUKANGXING(ANBIAN1:1000),QU15ml LIXINGUAN,JIARU5ml YIJIARUKANGXINGDEYETIPEIYANGJI。

  YONGXIAOBAITipsCONGPINGBANZHONGTIAOXUANDANKELONG,JIANGXIAOQIANGTOUDARU15ml LIXINGUANZHONG,30℃,180rpm,YAO >5h,YIKANDAOYETIPEIYANGJIBIANHUNZHUOWEIZHUN。

  QUGANJINGDE1.5ml EPGUAN,CONG15ml LIXINGUANZHONGXIQU1.5ml(ZHEIGELIANGKEGENJUJUNYEHUNZHUOCHENGDUJIAJIAN)JUNYE,LIXINJI12000rpm,LIXIN3min。

  QISHANGQING,JIARU250ul TIANGENZHILIXIAOTIKits(Cat# DP103-03,Lot# 6123)P1YE,ZHENDANG,CHEDIHUNYUN。

  JIARU250ul P2YE,WENHE(YIMIANDNADUANLIE)FANZHUAN6-8CI,SHIJUNTICHONGFENLIEJIE。(ZHEIYIBUZONGSHIJIANBUYAOCHAOGUO5min,FANGZHIGUODULIEJIE,ZHILISHOUDAOPOHUAI)

  XIANGEPGUANZHONGJIARU350ul P3YE,LIJIWENHESHANGXIAFANZHUAN6-8CI,CHONGFENHUNYUN,CISHIKEKANDAOBAISEXUZHUANGCHENDIAN,LIXINJI12000rpm,LIXIN3min。(P3JIARUHOUYINGLIJIHUNYUN,BIMIANCHANSHENGJUBUCHENDIAN,RUOSHANGQINGZHONGHAIYOUWEIXIAOBAISECHENDIAN,KEZAICILIXINHOUQUSHANGQING)

  JIANGXIFUZHUCP3(SHIYANQIANYONGPINGHENGYEBLCHULIXIFUZHUKEZUIDAXIANDUJIHUOGUIJIZHIMO,TIGAODELV;BLCHULIGUODEXIFUZHUZUIHAODANGTIANSHIYONG,FANGZHISHIJIANGUOZHANGHUIYINGXIANGXIAOGUO)FANGZAISHOUJIGUANZHONG,JIANGSHANGQINGZHUANYIDAOXIFUZHUCP3ZHONG,JINLIANGBUYAOXIDAOCHENDIAN,LIXINJI12000rpm,LIXIN30-60s,DAODIAOSHOUJIGUANZHONGDEFEIYE,JIANGXIFUZHUFANGRUSHOUJIGUAN。

  KEXUANBUZHOU:XIANGCP3ZHONGJIARU500ulQUDANBAIYEPD,12000rpm,LIXIN30-60s,DAODIAOSHOUJIGUANZHONGDEFEIYE,JIANGXIFUZHUFANGRUSHOUJIGUAN。(RUGUOSUZHUJUNSHIend A+ SUZHUJUNTG1、BL21、HB101、JMXILIE、ET12567DENG,YINQIHANYOUDALIANGHESUANMEI,SUOYITUIJIANCIBUZHOU;RUOWEIend A- SUZHUJUNDH5α、TOP10DENG,KESHENGLVECIBU)

  XIANGCP3ZHONGJIARU600ul PIAOXIYEPW(YIJIARUWUSHUIYICHUN),12000rpm,LIXIN30-60s,DAODIAOSHOUJIGUANZHONGDEFEIYE,JIANGXIFUZHUFANGRUSHOUJIGUAN。

  ZHONGFUBUZHOU9。

  LIXINJI12000rpm,LIXIN2min,MUDESHIJIANGXIFUZHUZHONGCANYUDEPIAOXIYEQUDIAO。

  JIANGCP3XIFUZHUFANGRUYIGEGANJINGDE1.5ml EPGUANZHONG,XIANGXIFUMODEZHONGJIANBUWEIDIJIA50-100ulXITUOYEEB,SHIWENFANGZHI2min,12000rpm,LIXIN2min,JIANGZHILIRONGYESHOUJIDAOEPGUANZHONG。(WEIZENGJIAZHILIDELV,KEJIANGRONGYEZAICIJIARUXIFUZHUCP3,SHIWENFANGZHI2min,12000rpm,LIXIN2min,JIANGZHILIRONGYESHOUJIDAOEPGUANZHONG;YEKEYITIQIAN65-70℃YUREEBYE)

  CENONGDU,PAOJIAOKANTIQUZHILIDEZHILIANG。

  ER.ZHILIDATI,CAOZUOZHEIYIBUQIANYIBANYAOZHILIXIAOTIQUERENZHILIDEZHILIANG。

  QUJIEJINGDEZHUIXINGPING,JIARUXIAOTIBUZHOU1ZHONGDELBYETIPEIYANGJI100ml,QUXIAOTIBUZHOU2ZHONGDEJUNYE10ul JIARUZHUIXINGPINGZHONG,30℃,180rpm,YAOGUOYE,YIKANDAOYETIPEIYANGJIBIANHUNZHUOWEIZHUN。

  BAOCUNJUNZHONG:YONG15%DEGANYOUBAOCUNJUNZHONG:1.5ml EPGUANZHONGJIARU350ul JUNYE+150ul 50%GAN,HUNYUN,-80℃BAOCUN。

  JIANGZHUIXINGPINGZHONGSHENGXIADEJUNYEYONG50ml LIXINGUAN,4℃,8000rpm,LIXIN5min,SHOUJIJUNTICHENDIAN,KETONGGUANDUOCI。

  GENJUSUOXUANDATISHIJIHEDESHUOMINGSHUANBUZHOUTIQU,BUTONGSHIJIHEBUZHOUBUYIYANG。

  TIQUJIESHUHOUCENONGDU,PAOJIAO(YINZHILIFENZILIANGYIBANJIAODA,SUOYIYONGDINONGDUDEQIONGZHITANGJIAOHEDADEmarker)

  YISHANGJIUSHIGUANYULIXINJIZAIZHILIDNATIQUZHONGDEYINGYONGZONGJIE,RUGUONINHAIXIANGLEJIEBOYACAIPIAOYOUGUANCHANPINDEYINGYONGXINXI,KEDUODUOGUANZHUSHANGHAILUXIANGYILIXINJIYIQIBOYACAIPIAO。


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